https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:12479 12 months or lack of menstruation for >4 months). CYP2C19 polymorphism was genotyped using PCR–RFLP method. Logistic regression was applied to assess CYP2C19 polymorphism as an effect modifier of cyclophosphamide. Seventy-one patients with SLE were enrolled, of which 36 (59.7%) had ovarian toxicity. CYP2C19*2 allele frequencies were 27.8 and 21.4% in the ovarian and non-ovarian toxicity groups. Patients with CYP2C19*1/*1 genotype and higher cumulative dose of cyclophosphamide (>23.75 g) had the highest odds of ovarian toxicity, i.e. 11.0 (95% CI: 1.2–99.1) times higher than patients with the CYP2C19*1/*2 or *2/*2 genotypes who received less cyclophosphamide (<23.75 g). After adjusting for age at start of treatment, this risk increased to 13.6 (95% CI: 1.1–162.2). Our results suggest that a cumulative cyclophosphamide dose of 23.75 g or higher carries a twofold higher risk of ovarian toxicity and the CYP2C19*1/*1 genotype increases the risk of toxicity a further fivefold.]]> Sat 24 Mar 2018 08:17:20 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:11520 th (Arg→Gly) and 27^th (Gln→Glu) amino acid positions of the β₂-Adrenergic receptor (ADRB2) may be linked to various asthma-related phenotypes. These include the adverse effects on lung function known to occur following the regular use of albuterol. The study aimed to determine the association between these two ADRB2 SNPs, their haplotypes and the phenotypes in Thai asthmatic patients. One-hundred and thirty asthmatic patients were genotyped at the Arg16Gly and Gln27Glu polymorphisms. Demographic data, disease severities, pulmonary function tests and medication usages were recorded for each patient. The frequencies of the Arg16 and Gln27 alleles were found to be 56.9% and 91.2%, respectively, while the linkage disequilibrium coefficient between the two SNPs was 0.36. Three haplotypes were estimated, i.e. Arg-Gln, Gly-Gln and Gly-Glu with frequencies of 148 (56.9%), 89 (34.2%) and 23 (8.9%), respectively. The mean percentages for predicted FEV₁ (%FEV₁) for these corresponding haplotypes were 73.5 (SD = 16.3), 72.4 (SD = 17.4) and 80.7 (SD = 13.1), respectively (p = 0.258). Additionally, the number of hospitalizations, emergency visits and inhaled corticosteroid/longacting β₂-agonist (ICS/ LABA) usages were lower in Gln/Glu subjects than for Gln/Gln genotyped patients, with values of 0% versus 11.9% (p = 0.122) for hospitalizations; 4.5% versus 18.8% (p = 0.121) for emergency visits; and 50% versus 76.6%, (p = 0.042) for ICS/LABA usages. The presence of the Glu27 allele in Thai asthmatic patients is associated with a decreased asthma severity, higher %FEV₁ values, less frequent hospitalizations and emergency visits, and decreased ICS/LABA usage.]]> Sat 24 Mar 2018 08:10:21 AEDT ]]>